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Wastewater Treatment
The United States Environmental Protection Agency (EPA) regulates and monitors the discharge of pollutants from municipal and industrial wastewater through the National Pollutant Discharge Elimination System (NPDES).

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National Pollutant Discharge Elimination System (NPDES)
The NPDES was established in 1972 under the Clean Water Act as a permit program to regulate pollutant discharge into the country’s water bodies. The EPA, except for Massachusetts, New Hampshire, New Mexico, the District of Columbia, U.S. territories, and federal and tribal lands, authorizes states to issue their NPDES permits based on EPA guidelines. The EPA identifies five categories of pollutants, and local and state agencies can set their limits based on the local environment’s needs.
Categories of Pollutants
- Oxygen-demanding substances (organic matter and ammonia)
- Pathogens
- Nutrients
- Inorganic and Synthetic Organic Chemicals (detergents and pharmaceuticals)
- Thermal pollution (when discharge water is higher in temperature than the receiving water)

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Sewage Treatment and Disinfection
The primary goal of sewage treatment is to remove contamination from human, bird, and animal feces. The authorities rely on testing for the presence of certain bacteria, known as ‘Fecal Indicator Bacteria (FIB)’ or ‘Indicator organisms’, to determine the safety of treated wastewater. Until recently, the test for drinking water safety relied on measuring the presence of fecal coliforms and fecal streptococci. However, a 2014 study estimated that globally, nearly 1.8 billion people suffered from fecal contamination in drinking water, partly due to faulty disinfection and the inadequacy of the existing FIB test.
The FDA determined that a dose of 120mJ/cm2 is required for medium-pressure UV technology to be considered equivalent to pasteurization. This dose is higher than for a 5-log reduction of common dairy contaminants (40mJ/cm2). Still, it is necessary because some viruses, like Adenovirus, are more UV-resistant. A 120mJ/cm2 dose ensures adequate virus inactivation.
Fecal Indicator Bacteria Testing
The EPA recommends testing Escherichia coli and Enterococcus bacteria to gauge drinking water safety. Despite this advancement, several issues still exist in testing for E. coli and Enterococci:
- E. coli does not survive for long outside the gut, making its testing more reliable closer to the point of contamination
- Measuring E. coli colonies reliably is challenging as other fecal bacteria easily outnumber it
- E. coli can be naturally found in non-contaminated samples depending on weather conditions
- Viable culturing of coliforms is difficult, leading to false negative test results
- Enterococci are naturally found in soil and sediments, making interpreting test results difficult
- The current tests do not capture significant pathogens such as Giardia, Cryptosporidium, Vibrio cholerae, and Salmonella
Despite these challenges, low cost, ease, and testing speed make E. coli and Enterococci testing famous for gauging adequate wastewater disinfection. The states of Texas and Maine have adopted Enterococci testing as a reliable mechanism for measuring sufficient disinfection. With new molecular test technologies like qPCR becoming mainstream, global standards may soon change, improving water safety and public health.
Disinfection Techniques
Chlorine and other oxidants have been proven effective in eliminating bacteria, but ultraviolet light (UV) has been gaining popularity. UV reduces human exposure to dangerous chemicals and the potential for accidental discharges that can harm aquatic life. The industry has evolved from the initial uses of chlorine, and alternative disinfection methods have been proven valuable in eliminating harmful microorganisms. These alternative methods eliminate material handling, mixing, or storage issues.
Further Reading:
- https://epd.georgia.gov/forms-permits/watershed-protection-branch-forms-permits/wastewater-permitting
- https://www.epa.gov/sites/production/files/2015-09/documents/primer.pdf
- Bain, R., Cronk, R., Hossain, R., Bonjour, S., Onda, K., Wright, J., . . . Bart, J. (2014, August). Global assessment of exposure to fecal contamination through drinking water based on a systematic review. Tropical Medicine and International Health, 19(8), 917-927. doi:10.1111/tmi.12334
- https://archive.epa.gov/water/archive/web/html/vms511.html
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